Cooling operation steps after Sterizaiton of Enoki bottle substrate 

Cooling operation steps after Sterizaiton of Enoki bottle substrate 

1. Cooling procedure
Confirm the cooling environment-confirm the sterilization procedure-open the cabinet door-confirm the temperature-exit the cabinet-place as required-close the cabinet door-confirm the equipment operation-confirm the material temperature before inoculation

2. Cooling operation steps
1. Confirm that the setting temperature of the cooling room is 14~15.5℃, the frequency of the evaporator fan, and the environment is clean and sterile.
2. Confirm that the sterilization procedure has been completed.
3. Close the interlayer door.
4. Open the cabinet door of the sterilizer that has completed the sterilization process according to the procedure.
5. Release heat and let the hot air in the cabinet door dissipate from the interlayer.
6. Confirm the air temperature after 15 minutes.
7. Exit the cabinet when the temperature is lower than 80℃.
8. Exit the cabinet from front to back in order, according to the wind direction (perpendicular to the direction of the evaporator fan)
9. After the No. 1 cooling room is full, place it in the No. 2 cooling room in order, first in the 28℃ cooling room.
10. Close the isolation doors of each area and cool.
11. Confirm the pressure, temperature setting, equipment operation, etc. of the cooling room
12. Check the material temperature before inoculation to determine whether it meets the requirements of inoculation.
13. After the inoculation work is completed, do a good job of sanitation and disinfection of the cooling room, buffer room, and interlayer.

Cooling process standards
① The way to get out of the pot. Above 80℃; directly enter the cooling room (without passing through the non-clean area)
② The cooling room (clean area) is clean and hygienic inside and outside.
③ Forced and rapid cooling.
④ The material temperature is cooled to 17~20℃ to prevent high temperature from inhibiting the growth of mycelium.